Accession Number:

AD0763351

Title:

Separation of Spleen Cells in Iso-Osmotic Ficoll Density Gradients.

Descriptive Note:

Scientific rept.,

Corporate Author:

ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD

Report Date:

1973-03-01

Pagination or Media Count:

25.0

Abstract:

Osmotic pressures of polymer solutions were examined as a function of density by equilibrium dialysis, and it was reaffirmed that the vant Hoff equation cannot be used to calculate the osmotic pressure of polymer solutions at concentrations greater than 5 percent. Therefore, it appears that previous methods utilizing polymer solutions for the generation of density gradients also generated hyperosmotic gradients. However, since cells maintain their integrity best in an isotonic solution, an iso-osmotic density gradient can be prepared by superimposing a reverse salt gradient on a Ficoll gradient. Using this method, the density profiles of several cell populations were determined, and observations on fractionated C57BL mouse spleen were made. Modified author abstract

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE