Effect of 5,5-Diphenylhydantoin (Dilantin) on Collagen Biosynthesis in Human Skin Fibroblasts.
Technical rept. Oct 69-Mar 71,
EDGEWOOD ARSENAL ABERDEEN PROVING GROUND MD
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Dilantin sodium in concentrations lower than .0001M does not influence collagen biosynthesis in stationary cultures of diploid human skin fibroblasts. At a concentration of .0001M Dilantin, cellular proliferation as measured by total culture protein is inhibited by approximately 50 as compared to controls. Both cellular proliferation and collagen biosynthesis are almost completely inhibited in the presence of .001M Dilantin. These findings suggest that Dilantin does not increase collagen biosynthesis by human fibroblasts in culture. They indicate that it is unlikely that increased wound collagen content reported in animals treated with Dilantin results from increased production of collagen by the fibroblasts at the wound site. Suthor