Biochemical Analysis of Cerebrospinal Fluid Changes in Response to Drug Administration.
Annual rept. no. 1, 1 May 72-1 May 73,
JOHNS HOPKINS UNIV BALTIMORE MD DEPT OF ENVIRONMENTAL MEDICINE
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The design of a chronic, remote sampling system for CSF with a total dead space of less than 0.06 ml is described. A monkey model was developed for the study of drug abuse while monitoring behavior, CSF neurochemical changes and cardiovascular effects of selected drugs. The first series of drug studies was completed using the d- and l-stereoisomers of amphetamine. Both isomers at levels of 1.5 mgkg effect the norepinephrine neuronal system as evidenced by the pronounced peripheral respiratory and cardiovascular effects. CSF levels of a dopamine metabolite HVA decreased significantly after the d- isomer indicating possible increased rates of release of dopamine, reduced reuptake of dopamine and increased possible o-methylation to 3-methoxy dopamine. Significant changes were observed in peripheral cardiovascular and respiratory systems which would be associated with norepinephrine neurons and which were produced by both amphetamine isomers with nearly equal effectiveness. Behavioral changes were of a type which indicate that dopamine neurons were selectively affected by the d- isomer and not the l- isomer. Modified author abstract