Factors Affecting the Level of Infectivity of RNA of Venezuelan Equine Encephalomyelitis (VEE) Virus
ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD
Pagination or Media Count:
RNA of the Venezuelan equine encephalomyelitis VEE virus, isolated from virions and infected cells was studied, and the main parameters for detection of maximal infectivity of the viral RNA were determined. It was shown that, among the factors determining the RNA infectivity level, adsorption time of the RNA on cells, the concentration and duration of treatment of the cells with NaCl, and the addition of polycations DEAE-dextran, protamine sulfate to the agar overlay, are all of significant importance. The data obtained in the study suggest optimal conditions for determining the infectious activity of the RNA of the VEE virus.