Mechanisms of Oxygen Toxicity at the Cellular Level.
MISSOURI UNIV COLUMBIA
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The following areas of research are summarized 1 Two types of pressure chambers were developed for exposure of bacteria and subcellular fractions to hyperbaric oxygen. 2 An electronic automated sampling device was developed to permit kinetic studies of biological transport as affected by hyperoxia. 3 The previously reported correlation between sensitivity to hyperoxia and radiation in bacteria was further studied. Evidence was presented that a small, but measurable amount of hyperoxic damage may occur through production of single-stranded breaks in DNA. 4 Surface SH groups on E. coli are oxidized at a rate of approximately 500 molecules per minute in 6 atm of oxygen, a condition which stops growth and respiration almost immediately. 5 Cellulose acetate membranes were discovered to inhibit growth of some strains of E. coli the significance of this for water analysis and for experiments utilizing membranes in HPO studies was discussed. 6 Fatty acid synthetase from yeast was purified approximately 150-fold and its sensitivity to HPO was measured. 7 HPO produced reversible inhibition of respiration and transport of glucose and acetate. Author Modified Abstract