Accession Number:

AD0758535

Title:

Structural Studies on the Specific Type VII Pneumococcal Polysaccharide,

Descriptive Note:

Corporate Author:

NATIONAL RESEARCH COUNCIL OF CANADA OTTAWA (ONTARIO) DIV OF BIOLOGICAL SCIENCES

Report Date:

1972-06-09

Pagination or Media Count:

12.0

Abstract:

The specific type VII pneumococcal polysaccharide was isolated from the crude capsular material by precipitative and chromatographic methods. It contained D-galactose, D-glucose, L-rhamnose, 2-acetamido-2-deoxy-D-glucose, and 2-acetamido-2-deoxy-D-galactose in the molar ratio of 3.52.33.02.11.0. Some of its structural features were revealed by methylation studies, time-lapse hydrolysis, periodate oxidation, and enzymic hydrolysis. The polysaccharide is branched at residues of D-galactose and 2-acetamido-2-deoxy-D-galactose. Non-reducing end groups consisted of D-galactopyranose and 2-acetamido-2-deoxy-D-glucopyranose residues, with the former predominating. Major components of the linear chains were 1 yield 3-linked L-rhamnose and 1 yield 4-linked D-glucose the minor ones were 1 yield 2-linked Lrhamnose, 1 yield 6-linked D-galactose, and 1 yield 6-linked 2-acetamido-2-deoxy-D-glucopyranose. The 1 yield 4-linked D-glucose components may be present as cellobiose residues. The results are in accord with structural features deduced from the serological cross-reactivity of this polysaccharide. Author

Subject Categories:

  • Biochemistry
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE