Wound Healing and Cellular Microenvironment.
Final technical rept.,
BRISTOL UNIV (ENGLAND)
Pagination or Media Count:
Intracellular measurements of pK, pNa and pH and extracellular monitoring of PO2 and PCO2 in fibroblasts showed that cells at the edges of wounds were easily damaged by hypoxia and shock. Irreversible changes involving mitochondria were correlated with an intracellular pH of 6.7 and falling membrane potential. Actively dividing cells in wounds were most susceptible to hypoxia than were synthesising or quiescent fibroblasts. Macrophages were much more tolerant of low intracellular pH. Low colloid osmotic fluid replacement in shock may raise blood pressure but fail to improve tissue oxygenation because of microedema. E. coli endotoxin, demonstrated by immuno-fluorescence, became localised in gut capillary endothelium and in liver Kupfer cells in early endotoxic shock. Clinical trials of the effects of maintaining pure O2 atmospheres locally over severely traumatised areas showed that this treatment can considerably improve tissue viability and healing time. A system was devised for measuring oxygen diffusion in normal and pathologic tissue. Author
- Anatomy and Physiology