A New Technique for Evaluating Antigenic Relatedness Among Viruses
Technical Report,01 Mar 1972,28 Feb 1973
MARYLAND UNIV COLLEGE PARK COLLEGE PARK United States
Pagination or Media Count:
The ability of the macrophage migration inhibition MMI test for differentiating both bacterial viruses and two cross-reacting strains of the avian coronavirus, infectious bronchitis virus. During the past year, the authors have demonstrated that Adenovirus types 1, 4, 5 and 7 are all clearly separable by MMI test procedures. It was also determined that the optimal sensitizing dose for Adenovirus type 1 was 100 micrograms per pig and that doses above or below this level gave poorer responses. In addition, two nuclear polyhedrosis viruses NPV which infect the alfalfa looper and cabbage looper two important insect pests respectively were clearly differentiated by MMI test procedures. The polyhedral sizes, shapes and virion occlusion patterns of the two species of NPV were indistinguishable. Attempts to passively transfer the delayed response to normal peritoneal exudate cells by using RNA extracts of tissues from sensitized animals have thus far been unsuccessful.