Syphilis Vaccine and Immune Mechanisms.
Annual rept. no. 1, 1 Apr 72-31 Mar 73,
CALIFORNIA UNIV LOS ANGELES DEPT OF MEDICAL MICROBIOLOGY AND IMMUNOLOGY
Pagination or Media Count:
Freshly isolated Treponema pallidum, Nichols strain, are being gamma-irradiated with a total of 650,000 rads, fortified with glycerol in final concentration of 15, and preserved in liquid nitrogen as a source of experimental vaccine. Rabbits have been immunized by both the intramuscular and intravenous routes at 3-week intervals with a total of 3 liquid nitrogen-preserved vaccine doses, each containing approximately 4 X 10 to the 9th power treponemes. Intradermal challenge has revealed some degree of acquired resistance. Each of the vaccinated animals developed VDRL antibody in contrast, only 3 of the 9 immunized by the intravenous route and none of those vaccinated intramuscularly developed TPI antibody. Treponema pallidum, Nichols strain, suspensions have been purified by multiple centrifugation techniques and appeared clean when examined by darkfield microscopy. Author
- Medicine and Medical Research