Research on Cell Biology of Cryoinjury: The Possible Role of Lysosomes in Cellular Injury After Freezing and Thawing.
Final progress rept. 1 Feb-1 May 71,
SAN FRANCISCO UNIV CALIF INST OF CHEMICAL BIOLOGY
Pagination or Media Count:
It is known that freezing and thawing can activate lysosomes by disrupting their membranes and releasing hydrolytic enzymes. As a result lysosomes may become harmful sites of catabolic reactions, thus adding more stress to an already damaged cell. Although it has been suspected that lysosomes might be implicated in cryoinjury, a systematic study on the possible effects of lysosomal enzymes on the fate of the cell following freezing and thawing is not available. This study was undertaken to assess to what degree, if any, activation of lysosomal enzymes correlates with the degree of cellular injury in response to certain variables of cryopreservation, and to investigate if agents that are known to stabilize lysosomal membranes could improve cell recovery.
- Anatomy and Physiology