Isolation of Snake Venom Toxins and Study of Their Mechanism of Action.
Final technical rept.,
ROGOFF-WELLCOME MEDICAL RESEARCH INST PETAH TIQWA (ISRAEL)
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The direct lytic factor DLF of cobra venom apparently modifies the red blood cell membrane, activating and eluting membrane-associated enzymes while having no effect on catalase. Several phospholipase A fractions obtained from Vipera palestinae and Naja naja venoms have been characterized homogeneity, amino acid composition, molecular weight and shown to possess both phospholipase A and B enzymic activity. Following intravenous injection of Echis coloratus venom, strong positive peroxidase activity can be demonstrated in endothelial cells, basement membrane and sometimes in the surroundings, of mouse brain capillaries and arterioles. A 6.8S immunoglobulin isolated from donkey anti-Russells viper venom and a 4.68S fraction obtained from it had neutralizing activity against both lethal and coagulating potencies of the venom. The specific neutralizing activities of both Igs were greater than that of the total globulins. Author