Accession Number:

AD0754823

Title:

Rapid Mixed Leukocyte Test with 3H-Acridine Orange1.

Descriptive Note:

Corporate Author:

CALIFORNIA UNIV SAN FRANCISCO MEDICAL CENTER

Personal Author(s):

Report Date:

1972-11-01

Pagination or Media Count:

11.0

Abstract:

The report concerns search for a tritium labeled compound which would maximally label lymphocytes in mixed culture MLC within 2 - 24 hours. For this purpose tritiated acridine orange 3H-AO was tested. Interest in acridine orange AO stemmed from the report of J. Andersson et al, that by cytophotofluorometric methods, an increase in fluorescence intensity of fixed and AO-stained lymphocytes can be observed 1-3 hours after human allogeneic lymphocytes have been committed to mixed culture. No such increase occurred in lymphocytes from donors compatible at the major histocompatibility locus HL-A. Data confirms that of Andersson et al., but by a method using liquid scintillation spectrophotometric measurement of 3H-acridine orange binding which is more available than estimation of bound acridine orange by spectrofluorometric fluorescence. Also, a method is described for the preparation of a pure tritiated acridine orange of high specific radioactivity.

Descriptors:

• (*LYMPHOCYTES
• IN VITRO ANALYSIS)
• (*LEUKOCYTES
• IN VITRO ANALYSIS)
• LABELED SUBSTANCES
• TRITIATED COMPOUNDS
• ACRIDINES
• DYES
• CYTOLOGY
• IMMUNOLOGY
• FLUORESCENCE
• HISTOLOGICAL TECHNIQUES

Subject Categories:

• Anatomy and Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE