Accession Number:

AD0754337

Title:

Indirect Hemagglutination Test to Detect Antibodies for Tickborne Encephalitis Virus,

Descriptive Note:

Corporate Author:

ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FREDERICK MD

Personal Author(s):

• Margolin,R. D.
• Kondrashov,Z. N.

Report Date:

1972-01-01

Pagination or Media Count:

8.0

Abstract:

The possibility of using the indirect hemagglutination test IHA with native goose erythrocytes is utilized in the detection of antibodies for tick-borne encephalitis virus. The relationship between the antibody titers in the IHA test and conditions of sensitization and performance of the reaction are studied. Sensitization is achieved by incubating a 2 suspension of erythrocytes in a phosphate buffer 5.8 - 6.0 pH with an equal volume of antigen for a period of 15 minutes at 37 degrees. The sensitized erythrocytes should be added to the serum solutions in the form of an 0.33 suspension in a dextrose-gelatine-veronal buffer. The reaction may take place at 4 degrees or at room temperature. The IHA is specific and more sensitive than the presently used HI test. Author

Descriptors:

• *SERODIAGNOSIS
• GROUP B ARBOVIRUSES
• ANTIGENS
• ANTIBODIES
• ARBOVIRUSES
• USSR

Subject Categories:

• Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE