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Indirect Hemagglutination Test to Detect Antibodies for Tickborne Encephalitis Virus,
ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FREDERICK MD
Pagination or Media Count:
The possibility of using the indirect hemagglutination test IHA with native goose erythrocytes is utilized in the detection of antibodies for tick-borne encephalitis virus. The relationship between the antibody titers in the IHA test and conditions of sensitization and performance of the reaction are studied. Sensitization is achieved by incubating a 2 suspension of erythrocytes in a phosphate buffer 5.8 - 6.0 pH with an equal volume of antigen for a period of 15 minutes at 37 degrees. The sensitized erythrocytes should be added to the serum solutions in the form of an 0.33 suspension in a dextrose-gelatine-veronal buffer. The reaction may take place at 4 degrees or at room temperature. The IHA is specific and more sensitive than the presently used HI test. Author
APPROVED FOR PUBLIC RELEASE