Development of Automated Methods for Detection of Anti-Globulins and Measurement of Immunoglobulin Antigens.
Final comprehensive rept.,
CORNELL UNIV MEDICAL COLL NEW YORK DEPT OF MEDICINE
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Studies were carried out to determine the characteristics and reproducibility of automated agglutination methods for the detection of immune reactions between gamma G-globulin coated red cells and antiglobulin antibodies. An inhibition of hemagglutination assay for gamma G antigens was graphed as a regression line of inhibition with a characteristic slope. The effects of heat denaturation, changes in red cell concentration, alterations of antibody titer, and cross reacting antigenic determinants were described, and the most suitable conditions for measurements of the gamma G-antigens discussed. The use of discrete samples vs. increasing gradients of inhibitor was tested and it was concluded that in continuous flow analysis, gradients were unsuitable because of sample carry-over. It was concluded that the automated agglutination assay for gamma G globulins was a sensitive and potentially productive approach to the detection and analysis of immune reactions. Author
- Medicine and Medical Research