Accession Number:

AD0752178

Title:

Isolation of Infectious RNA from Certain Group A and Group B Arboviruses,

Descriptive Note:

Corporate Author:

ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FREDERICK MD

Report Date:

1972-01-01

Pagination or Media Count:

6.0

Abstract:

The infectious RNA may be isolated with cold phenol from Japanese encephalitis virus. Treatment with hot phenol is necessary for isolation of RNA of Venezuelan equine encephalomyelitis virus. Removal of residual phenol with ether and nitrogen increases the yield of infectious RNA approximately 10-fold as compared with alcohol reprecipitation. A considerable increase in RNA titers results from dilution of preparations for inoculation of ceil cultures in tris buffer with 1 M NaCl solution, pH 7.8 and washing of cultures with hypertonic solution before inoculation. The yield of VEE virus RNA was up to 0.25 of infectivity of the original virus. Author

Subject Categories:

  • Biochemistry
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE