Extracellular Synthesis of Infectious Ribonucleoprotein on Subcellular Structures
ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD
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The synthesis of the infectious ribonucleoprotein virus was effected in the mitochondrial-microsomal MM fraction isolated from the chicken fibroblast cells infected with the virus of the Venezuelan form of equine encephalomyelitis VFEE 2 12 - 3 hours from the moment of inoculation and then incubated in the N199 medium or in the system for defining the polymerase activity. Following incubation of the said system for 3-4 hours the infection titre of the product increased 75-100-fold by comparison with the initial one. It is shown that such rise of the infectiousness had been caused not by elution of the residual virus with the subcellular structures into the medium, but by the synthesis of the infectious material de novo. In this system both the single- and two-band RNA in synthetized, being then associated with virus- specific and cellular proteins and forming ribonucleoprotein complexes. The latter possess infectious properties and are partly neutralized with the virus- specific serum.