Accession Number:

AD0751328

Title:

Investigation of Chain Reactions and Oxygen Effects During Radiolysis of Peptide Disulfide Bonds Using Cysteine-Glutathione Disulfide as a Model,

Descriptive Note:

Corporate Author:

NATIONAL RESEARCH COUNCIL OF CANADA OTTAWA (ONTARIO) DIV OF BIOLOGY

Personal Author(s):

Report Date:

1971-06-07

Pagination or Media Count:

11.0

Abstract:

Gamma-radiolysis of cysteine-glutathione disulfide, the mixed disulfide of cysteine and the tripeptide glutathione, has been examined in unbuffered aqueous solutions 0.3 mM. In the presence of air the principal products are sulfinic and sulfonic acids of cysteine and glutathione and the symmetrical disulfides, cystine and glutathione disulfide. In the absence of air, cysteine, glutathione, and the sulfinic acid derivatives of these thiols were produced. Irradiation of solutions containing various concentrations of mixed disulfide and a range of oxygen concentrations showed that the high yields of symmetrical disulfides were due to a chain reaction which could be suppressed by oxygen. Author

Subject Categories:

  • Biochemistry
  • Radiobiology
  • Radiation and Nuclear Chemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE