Accession Number:

AD0750637

Title:

The Effect of pH and Inosine on the Regeneration of ATP, 2,3-DPG and P(50) of Blood Incubated at 1C. and 37C.

Descriptive Note:

Annual summary rept. no. 69, 16 Oct 71-30 Sep 72,

Corporate Author:

BRYN MAWR HOSPITAL PA

Personal Author(s):

Report Date:

1972-10-01

Pagination or Media Count:

9.0

Abstract:

The technical objective of the work reported was to develop practical methods to maintain the highest level of 2,3-DPG and oxygen dissociation along with a reasonable survival. ATP values of 69 plus or minus 2 compatible with 70 survival were achieved with any of the methods reported. Best function, as reflected by 2,3-DPG and P50 values, was achieved when blood was collected into ACD pH 5.6 and incubated at 37C. with inosine at pH 7.0 or 7.2. Author

Subject Categories:

  • Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE