The Effect of pH and Inosine on the Regeneration of ATP, 2,3-DPG and P(50) of Blood Incubated at 1C. and 37C.
Annual summary rept. no. 69, 16 Oct 71-30 Sep 72,
BRYN MAWR HOSPITAL PA
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The technical objective of the work reported was to develop practical methods to maintain the highest level of 2,3-DPG and oxygen dissociation along with a reasonable survival. ATP values of 69 plus or minus 2 compatible with 70 survival were achieved with any of the methods reported. Best function, as reflected by 2,3-DPG and P50 values, was achieved when blood was collected into ACD pH 5.6 and incubated at 37C. with inosine at pH 7.0 or 7.2. Author