Acetylcholinesterase Substrates: Acetoxymethylpyridines and Benzyl Acetate.
Technical rept. Jul 71-Jan 72,
EDGEWOOD ARSENAL MD
Pagination or Media Count:
2-Acetyloxymethylpyridine compound I and 2-, 3-, and 4-acetoxymethylpyridinium methiodides compounds II, III, and IV are spectrophotometrically useful substrates for acetylcholinesterase. Compounds III and IV are highly water soluble, yet equal to phenyl acetate in resistance toward aqueous hydrolysis. Compound I and benzyl acetate are appreciably more stable. Kinetic constants for both enzymatic and nonenzymatic hydrolysis are reported. Comparison of the relative rates of acylation of acetylcholinesterase by compounds I, III, and IV are phenyl acetate indicates considerable kinetic selectivity. Contrary to general expectations, the uncharged compounds, I and phenyl acetate, have the highest turnover rates. Author