Metabolism of Cell Fragments of 'Neisseria meningitidis' with Special Emphasis on Carbohydrate Synthesis.
Medical research progress rept. no. 14, 1 Apr 71-30 Jun 72,
NAVAL MEDICAL RESEARCH INST BETHESDA MD
Pagination or Media Count:
The report describes two studies of fractions of group B N. meningitidis. Spheroplast membranes spheroplast envelopes of strain 2091 of group B Neisseria meningitidis were prepared by a procedure that included lysozyme treatment of the cells and osmotic lysis of the resulting spheroplasts. The membrane preparation consisted of 62 protein, 28 lipid, 9 ribonucleic acid, small amounts of carbohydrate, hexosamine, and deoxyribonucleic acid. Succinate dehydrogenase and reduced nicotinamide adenine dinucleotide NADH oxidase activities were found to be associated with the spheroplast membranes. N-acetylneuraminic acid NAN - condensing enzyme and cytidine monophosphate-NAN synthesizing enzyme also were identified in this organism. For preparation of cytoplasmic membrane-enriched fractions, N. meningitidis group B cells were fractionated by the Schnaitman technique which consists of disrupting the bacteria with a French pressure cell and separating the fragments by sucrose gradient centrifugation. Three bands were isolated the two lighter bands exhibited reddish brown color characteristics and succinic dehydrogenase SDH activity. The heavier white band contained predominately cell wall material and consisted of one to five layers. Author