Blood Preservation Solutions. XI. Raising the pH to Improve Hemoglobin Function.
ARMY MEDICAL RESEARCH LAB FORT KNOX KY
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The effects of pH increase on hemoglobin function in a standard blood preservative solution during blood storage were assessed. Established methods were used for collecting and storing blood under blood banking conditions, assaying red cell 2,3-diphosphoglycerate 2,3-DPG--a measure of hemoglobin function, and adenosine triphosphate ATP--a measure of red cell viability, as developed previously in this laboratory. Increasing the pH by small gradual increments of the commonly used preservative, acid-citrate-dextrose ACD, results in a significant improvement in hemoglobin function during the first week of liquid storage at 4 C. Better maintenance of hemoglobin function, as determined by red cell 2,3-DPG concentrations, was observed with preservatives at higher pH levels. On the other hand, at the pH ranges employed in this study, ATP levels were not greatly different over the storage period considered. Author