Uptake and Retention of Adenine Moiety by Stored Human Red Blood Cells.
ARMY MEDICAL RESEARCH LAB FORT KNOX KY
Pagination or Media Count:
The adenine moiety, which is utilized by the red cells for net synthesis of various nucleotides, does not appear to be in a steady-state equilibrium between plasma and erythrocytes. As storage time increases, the equilibrium is shifted towards the red cells and reached a maximum radioactive content at 21 days. After this time a reversal occurs. At the beginning of storage the association of adenine with erythrocytes is not very strong, since the radioactive material can be removed to a great extent by saline extraction. The retention increases with length of storage and attains a maximum value at 21 days. Specific association of the radioactive label has been observed with cell populations of low density young cells. Author