Accession Number:

AD0748549

Title:

Using Scrapings from Formalin-Fixed Tissues to Diagnose Leptospirosis by Fluorescent-Antibody Techniques

Descriptive Note:

Corporate Author:

ARMED FORCES INST OF PATHOLOGY WASHINGTON DC

Report Date:

1971-01-01

Pagination or Media Count:

5.0

Abstract:

Small specimens of formalin-fixed tissues approximately 1 x 1 x 0.2 cm were cut from the suspect speciment. Several clean microscope slides were dipped in 1 aqueous gelatin and air-dired or dried on a slide warmer. Each tissue specimen was washed in running tap water for 2-5 min and then lightly scraped with a straight knife blade, cutting edge perpendicular to the surface of the speciment. The scrapings were allowed to build up and cling to the knife blade, which was then turned so that the broad surface contacted the slide thus, the scrapings could be smeared onto the slide in a single motion. Sufficient pressure was applied to embed the tissue fragments in the gelatin coating. Smears, dried in air or on a slide warmer, were stained immediately by a standard direct or indirect technique to detect fluorescein-labeled antigens. This scraping method, adapted to the study of leptospirosis by fluorescent- antibody technique, could reduce the need for cryostat-cut tissues and facilitate the observation of individual leptospires.

Subject Categories:

  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE