The Analysis of L-3-Hydroxytyrosine (L-dopa) and its Metabolites
Final rept. May 1969-Jun 1971
AIR FORCE AEROSPACE MEDICAL RESEARCH LAB WRIGHT-PATTERSON AFB OH
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A procedure for the extraction and analysis by gas-liquid chromatography of a variety of catecholamine metabolites from biological tissue was developed. An extensive investigation and evaluation of extraction techniques was undertaken. Catecholamine reactions which would result in stable, volatile derivatives suitable for separation and measurement by gas-liquid chromatography were also investigated. Since the detection by electron capture provided the greatest sensitivity in analysis for certain compounds, special consideration was given to reactions which would form derivatives suitable for this type detection. Because of their stability, volatility, and electron affinity, the pentafluoropropionyl derivatives were selected as the derivatives of choice. The rapidity of the technique and the advantage of using small sample volumes should make it applicable for the solution of a wide variety of problems in both research and clinical laboratories.