Accession Number:

AD0745659

Title:

'Pseudomonas aeruginosa' Exotoxin: Effect on Cell Cultures

Descriptive Note:

Medical research progress rept. no. 1,

Corporate Author:

NAVAL MEDICAL RESEARCH INST BETHESDA MD

Report Date:

1972-01-31

Pagination or Media Count:

10.0

Abstract:

An exotoxin, toxic to both mice and cultured cells, was isolated from cultures of Pseudomonas aeruginosa. Relatively small amounts of the exotoxin inhibited the uptake of uridine and amino acids by Vero cells. Within limits, this toxic action was reversible and could be inactivated by heating at 70 C or by proteolytic digestion, but it was not affected by nucleases. The inhibitory activity of the toxin could also be decreased by rabbit antiserum. Metabolic activities of Vero cells, generally associated with the production of energy, did not appear to be affected by the toxin.

Subject Categories:

  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE