'Trypanosoma lewisi': An in vitro Model System for the Study of the Effects of Humeral Factors on Growing Parasitic Protozoa.
Final progress rept.,
KANSAS UNIV LAWRENCE DEPT OF MICROBIOLOGY
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Techniques developed for the maintenance and increased production of T. lewis have been modified and utilized for other lewisi group typanosomes. The antigenic relationships between culture forms CF and Blood Stream forms BSF have been established during cultivation and infection. All antigens were proteins and serological cross-reactions were detected with other lewisi group trypanosomes. Resistance was dependent on the age, sex and the physiological state of the host. Young animals were more susceptible than old and females more susceptible than males. Depending on the time of infection and conception, pregnant animals showed more or less severe parasitemias. Survival was also dependent on this relationship. Both 19S and 7S Ig were synthesized during T. lewisi infections. Agglutinintiters were higher to dividing than to non-dividing BSF. Ablastin was found in the 7S fraction. ALS treatment of animals before and after infection indicated ablastin was an antibody and differed from the agglutinins. Immunization with somatic extracts of CF and BSF also elicited immunity to reinfection. Protective immunity was correlated with a marked agglutinin response. Author