Accession Number:

AD0744805

Title:

The Reaction of Tris-(Choline Chloride)Phosphate with Eel Cholinesterase

Descriptive Note:

Technical rept. Nov 1970-Dec 1971

Corporate Author:

EDGEWOOD ARSENAL ABERDEEN PROVING GROUND MD

Personal Author(s):

• Amshey, Jr., Joseph W.
• Steinberg, George M.

Report Date:

1972-06-01

Pagination or Media Count:

17.0

Abstract:

Tris-choline chloridephosphate TCCP has been observed to slowly and progressively inhibit eel acetylocholinesterase. The kinetics of the inhibition reaction are consistent with a mechanism involving binding of the inhibitor to the enzyme, followed by a rate-limiting irreversible reaction producing inactive enzyme. The dissociation constant of the enzyme-inhibitor complex and the rate constant of the inactivation reaction have been determined. The results obtained with TCCP are compared with available data on the relative enzymic reactivity of alkoxy and thioloesters of phosphoric acid.

Descriptors:

• *CHOLINESTERASE INHIBITORS
• *ORGANIC PHOSPHORUS COMPOUNDS
• REACTION KINETICS
• ENZYMES
• PHOSPHATES
• PHOSPHORYLATION
• CHOLINES
• AMMONIUM COMPOUNDS
• ACETYLCHOLINESTERASE

Subject Categories:

• Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE