Sensitization of Deoxyribonucleic Acid to Endonuclease by Adenine Alkylation.
Technical rept. Jul 69-Jun 71,
EDGEWOOD ARSENAL MD
Pagination or Media Count:
Monofunctional sulfur mustards inactivated intracellular T1 bacteriophage in an exponential manner when treated complexes were plated in the presence of acriflavine. At 37 survival, the virus had sustained one adenine and six guanine alkylations at the N3 and the N7 positions, respectively. On the other hand, a monofunctional nitrogen mustard, which alkylated guanine but not adenine, had no effect on the virus. Although depurination following alkylation was too slow to account for the loss of biological activity, sensitization to a nuclease appeared to play a role. Only those agents that inactivated T1 phage were capable of alkylating adenine and thereby sensitizing the polymer to an endonuclease. The importance of adenine aklylation was further elucidated in studies employing synthetic polydeoxyribonucleotides. Author