The Rapid Diagnosis of Acute Respiratory Disease Due to Adenovirus: Preparation and Standardization of Fluorescent Antibody Reagents
Medical research progress rept. no. 6
NAVAL MEDICAL FIELD RESEARCH LAB CAMP LEJEUNE NC
Pagination or Media Count:
Fluorescent antibody reagents were prepared for the rapid diagnosis of acute respiratory disease caused by adenovirus in a military population. Methods are described for the preparation of high-titered antisera in rabbits, fractionation of globulins from the serum, conjugation with fluorescein isothiocyanate, and sorption to remove nonspecific staining. Fluorescein-tagged antisera were specific at diluations of 120 to 130.
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