Accession Number:

AD0742202

Title:

Automated Methods for Measuring Blood Cholinesterase: A Selective Comparison and a Novel Artifact

Descriptive Note:

Technical rept. Feb 1970-Jun 1971

Corporate Author:

EDGEWOOD ARSENAL ABERDEEN PROVING GROUND MD

Report Date:

1972-04-01

Pagination or Media Count:

17.0

Abstract:

Cholinesterase activity units obtained by the more commonly used Levine automated systems are not valid, as the standard calibration curve for the method is determined with an artificial standard--glutathione, either in the presence or absence of the substrate acetylthiocholine. In the presence of the substrate, glutathione reacts with a substrate via a transesterification mechanism. The slope of the calibration curve, and consequently activity units, would vary, depending on the rate of interaction. In the absence of substrate, as glutathione dialyzes about one-third as rapidly as thiocholine, activity units are obtained that are approximately three times greater than valid values. An acceptable automated procedure is recommended. Utility and sensitivity of selected automated cholinesterase systems are discussed.

Subject Categories:

  • Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE