Accession Number:

AD0741816

Title:

Specificity of a Rabbit Antihuman Lymphocyte Serum.

Descriptive Note:

Progress rept.,

Corporate Author:

ARMY MEDICAL RESEARCH LAB FORT KNOX KY

Personal Author(s):

Report Date:

1972-01-28

Pagination or Media Count:

22.0

Abstract:

The specificity of antibodies produced after prolonged immunization of rabbits with purified human lymphocytes from a single individual B.L. was examined. Lymphocytes and leukocyte-platelet packs from B.L. and from ten other individuals unrelated to B.L. and to each other were used. The antiserum had a cytotoxicity titer of 1256 against B.L. lympnocytes and against lymphocytes from eight other individuals. A titer of 1128 was obtained against lymphocytes from the other two individuals. Absorption studies at 164 and 14 dilution of the antiserum indicated that separate absorptions with purified lymphocytes 164 or leukocyte-platelet packs 14 from each of the ten other individuals, as well as from B.L., completely removed cytotoxicity against both the absorbing lymphocytes and B.L. lymphocytes with one exception at the 14 dilution. Absorption studies at the 164 dilution indicated that residual cytotoxicity existed for B.L. lymphocytes at the point where cytotoxicity was just removed against the absorbing lymphocytes from each of the ten other individuals. Author

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE