Utilization of (32P) Soman for Measurement of Acetylcholinesterase in Brain Tissues
Special publication Nov 1967-Jul 1969
EDGEWOOD ARSENAL ABERDEEN PROVING GROUND MD
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Acetylcholinesterase acetylcholine hydrolase, EC 220.127.116.11 activity in brain tissues of the dog was measured before and after inhibition with 32P- labeled pinacolyl methylphosphonofluoridate Soman. A high correlation was obtained between uninhibited enzyme activity and soman-derived radiophosphorus bound to the tissues as methyl 32P phosphonate. The methylphosphonate for 100 inhibition was taken equivalent to the catalytic sites. Turnover numbers calculated from the ratio of acetylcholine hydrolysis to catalytic sites approached values yielded by eel acetylcholinesterase.