A New Technique for Evaluating Antigenic Relatedness among Viruses.
Rept. no. 1 (Annual), 1 Mar 71-29 Feb 72,
MARYLAND UNIV COLLEGE PARK DEPT OF MICROBIOLOGY
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The macrophage migration inhibition MMI test was evaluated for its ability to identify selected bacterial and animal viruses. The use of both purified viruses and a dual propagation system for viruses was studied. The P22 and P221 phage of Salmonella typhimurium were clearly distinguished from each other by the MMI test with good statistical significance. In addition, a dose response curve for migration inhibition caused by increasing concentrations of P22 in the test system was established. Excellent results have also been obtained with several Adenoviruses. The use of cells from guinea pigs sensitized to either purified Adenovirus types 1 or 4 yielded MMI tests which were highly specific for the homologous antigen virus tested against Adenoviruses 1, 4, 5 and 7. The MMI test was able to distinguish between crossreacting infectious bronchitis virus IBV strains using a dual system for propagating IBV. Following guinea pig immunization with chicken tracheal explant grown IBV-1, the MMI test was able to differentiate IBV-1 from IBV strains 8 and 10 using embryonated egg propagated virus. Author