Accession Number:
AD0739626
Title:
A Spin Label Study of the Organization and Fluidity of Hydrated Phospholipid Multibilayers - a Model Membrane System,
Descriptive Note:
Corporate Author:
NATIONAL RESEARCH COUNCIL OF CANADA OTTAWA (ONTARIO) BIOCHEMISTRY LAB
Personal Author(s):
Report Date:
1971-01-01
Pagination or Media Count:
12.0
Abstract:
The essential theory of the spin label method is presented and applied to the case of a rigid spin label localized in an oriented phospholipid smectic mesophase hydrated phospholipid multibilayers. The fatty acid chains of the phospholipids in a model membrane of egg lecithin are shown to make an average angle of 24 deg with respect to a perpendicular to the bilayer plane. Addition of cholesterol stiffens the bilayers and decreases this angle. The various requirements of a sterol for this condensing effect are explored. Ions, proteins, anesthetics, antibiotics, and decouplers of oxidative phosphorylation are shown to have profound effects on the structure of lipid bilayers. Author
Descriptors:
Subject Categories:
- Biochemistry