Accession Number:

AD0739626

Title:

A Spin Label Study of the Organization and Fluidity of Hydrated Phospholipid Multibilayers - a Model Membrane System,

Descriptive Note:

Corporate Author:

NATIONAL RESEARCH COUNCIL OF CANADA OTTAWA (ONTARIO) BIOCHEMISTRY LAB

Personal Author(s):

Report Date:

1971-01-01

Pagination or Media Count:

12.0

Abstract:

The essential theory of the spin label method is presented and applied to the case of a rigid spin label localized in an oriented phospholipid smectic mesophase hydrated phospholipid multibilayers. The fatty acid chains of the phospholipids in a model membrane of egg lecithin are shown to make an average angle of 24 deg with respect to a perpendicular to the bilayer plane. Addition of cholesterol stiffens the bilayers and decreases this angle. The various requirements of a sterol for this condensing effect are explored. Ions, proteins, anesthetics, antibiotics, and decouplers of oxidative phosphorylation are shown to have profound effects on the structure of lipid bilayers. Author

Subject Categories:

  • Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE