'In vivo' Role of 'Pseudomonas aeruginosa' Toxins and Host Response
Annual rept. no. 1, 1 Mar 1971-29 Feb 1972
WAYNE STATE UNIV DETROIT MI DEPT OF IMMUNOLOGY AND MICROBIOLOGY
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Five standard methods of extracting endotoxin were employed in an effort to establish its presence in Pseudomonas aeruginosa as well as to make comparative evaluations of its biological and chemical properties. Of the five preparations, aqueous phenol extracted endotoxin exhibited the greatest degree of lethality. The LD50 was 450 micrograms dry weight when administered intravenously and 840 micrograms intraperitoneally. No lethality was observed when endotoxin was administered intranasally. Lethality appeared to be associated with the core region of the lipopolysaccharide molecule, while no correlation between lethality and lipid content was detected.