Accession Number:

AD0737602

Title:

An Evaluation of Several Methods of Processing Plants for Autoradiography

Descriptive Note:

Technical memo.

Corporate Author:

FORT DETRICK FREDERICK MD

Report Date:

1971-08-01

Pagination or Media Count:

27.0

Abstract:

Because of the possible errors in interpretation as a result of the occurrence of pseudoimages formed by improper preparation of plant material for autoradiography, three drying methods were compared freeze-drying, oven-drying, and air-drying. Freeze-drying was the best method for preparing plants for autoradiography, oven-drying the least effective method. Pseudoimages were consistently produced by oven-drying, especially in bean plants these false images were particularly characterized by peripheral movement of the label in the treated leaves. Sectioning did not prevent this movement. Air-drying was ranked second as an effective technique. Pseudo-images produced by air-dried plants appeared to be relatively minor compared with those of oven-dried plants. The apparent presence of the label in the roots appeared to be the principal anomaly in air-dried bean plants. Freeze-drying was not as essential for the woody plants as for the bean plants. Air-dried ash produced excellent autoradiographs similar to those of freeze-dried ash. However, metabolic degradation of the labeled compound may occur during air-drying, resulting in probable loss of the one-for-one relationship between the compound under study and the radioactive tracer.

Subject Categories:

  • Agronomy, Horticulture and Aquiculture
  • Biology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE