Ultraviolet Light Induced Fluorescence and Phosphorescence of Bacteria at 90 Kelvin.
NAVAL DENTAL RESEARCH INST GREAT LAKES ILL
Pagination or Media Count:
Ultraviolet light induced fluorescence and phosphorescence of washed bacterial cells at 90K was due to tryptophan in cell protein with some contributions from nicotinamide adenine dinucleotide and tyrosine. Gram-negative bacteria showed similar emissivites in cells and walls. Gram-positive bacterial lacked phosphorescence in walls consistent with the absence of complete protein. All intact cells absorbed light at wavelengths greater than 256 nm and dissipated it via available singlet and triplet states and by energy migration among the various luminscent groups. Those results broaden the understanding of interactions between radiant energy and bacteria. Author