Final Report Office of Naval Research Contract N00014-68-C-0209, 1970
Rept. for 1 Nov 1967-30 Oct 1970
BIOMEDICAL RESEARCH INST ROCKVILLE MD
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The work performed dealt with prolonged and short-term perfusion of mammalian hearts of normothermic and hypothermic temperatures and with infusion of hypertonic cell free fluids into animals in hemorrhagic shock. In addition some aspects of freeze preservation of cardiac tissues and microvascular injury in perfusion were studied. It was demonstrated in the course of the above investigations that excised primate hearts can be resuscitated by pulsatile perfusion with cell free fluids as long as 2 hours postmortem. Infusion of cell free solutions of various tonicities into animals in hemorrhagic shock demonstrated an extremely rapid adjustment of osmolality of the peripheral blood and the interstitial fluid. Studies with freezing of tissues were concentrated on perfusion with cryoprotective agents and to freezing of pieces of myocardium, whole hearts peripheral blood leukocytes and bone marrow.
- Medicine and Medical Research