Accession Number:

AD0733824

Title:

Development and Substantiation of the Principles of Obtaining Large Amounts of Interferon

Descriptive Note:

Corporate Author:

FORT DETRICK FREDERICK MD

Personal Author(s):

Report Date:

1971-12-07

Pagination or Media Count:

8.0

Abstract:

The optimum conditions for the formation of interferon, induced by Group A arboviruses VEE and Sindbis viruses, in primary trypsinized chick embryo fibroblast cultures, using various methods of cultivation roller, and concentration of cells, in terms of 1 ml of the medium, are the most important factors determining the level of interferon production. Multiplicity of infection is important, mainly when suspension is used, and is optimally 1 - 10 PFU.A rise in the incubation temperature brings about an increase in the production of interferon, induced by the Sindbis virus.

Subject Categories:

  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE