Development and Substantiation of the Principles of Obtaining Large Amounts of Interferon
FORT DETRICK FREDERICK MD
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The optimum conditions for the formation of interferon, induced by Group A arboviruses VEE and Sindbis viruses, in primary trypsinized chick embryo fibroblast cultures, using various methods of cultivation roller, and concentration of cells, in terms of 1 ml of the medium, are the most important factors determining the level of interferon production. Multiplicity of infection is important, mainly when suspension is used, and is optimally 1 - 10 PFU.A rise in the incubation temperature brings about an increase in the production of interferon, induced by the Sindbis virus.