The Effect of DEAE-Dextran, Protamine Sulfate and Sulfated Polysaccharides of Agar on Multiplication of Venezuelan Equine Encephalomyelitis Virus in Cell Cultures
FORT DETRICK FREDERICK MD
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The model of VEE virus and trypsinized chick embryo cells was used to study the effect of diethylaminoethyl-dextran DEAE-D, agar polysaccharides APS and protamine sulfate PS on the pattern of virus-cell interaction. The presence in the growth medium of DEAE-D and PS inhibited virus production. The inhibiting effect of DEAE-D was manifested in the first stages of virus-cell interaction and was more marked with increasing pH. Sulfated polyanions of agar inhibited virus multiplication affecting later stages of reporduction. Lowering of pH of the growth medium activated the inhibiting effect of polysaccharides.