Accession Number:

AD0733394

Title:

Virus-Like Particles Formed in Vivo and in Vitro,

Descriptive Note:

Corporate Author:

FORT DETRICK FREDERICK MD

Report Date:

1971-11-18

Pagination or Media Count:

12.0

Abstract:

Ribonucleoprotein complexes possessing infectivity and capable of producing typical plaques under agar were formed in mitochondrial fraction isolated from cells infected with Venezuelan equine encephalomyelitis virus VEE in the course of incubation in appropriate media. In the linear sucrose gradient, sedimentation constants of the indicated complexes were from 80S to 160S, and their bouyant density in CsCl gradient varied from 1.30 gcc to 1.42 gcc. Virus-like particles pseudoviruses with similar characteristics were found after addition of the infectious RNA of VEE virus to homogenate obtained from uninfected cells. They were partially resistant to robonuclease and could not be neutralized by virus-specific serum. It is suggested that the basis of the formation of virus-like particles in vivo and in vitro systems is the complexing between viral RNA and cell proteins. Here formation of structures of the type of informosomes may occur. Author

Subject Categories:

  • Biochemistry
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE