Accession Number:

AD0733311

Title:

Biodetection of Microbial Contamination in Processed Foods: The Potential for Development of Odorous Excretory Materials in Animals Fed Rations Containing Pyridine-N-Oxides

Descriptive Note:

Final rept. May 1970-May 1971

Corporate Author:

MIDWEST RESEARCH INST KANSAS CITY MO

Personal Author(s):

Report Date:

1971-08-01

Pagination or Media Count:

37.0

Abstract:

Certain pyridine-N-oxides have been advocated for use as biological contamination detectors in processed foods. Through a bioreduction brought about by the presence of bacteria a nonodorous heterocyclic N-oxide substrate is converted to an odorous pyridine compound. The odor is used as a signal indicating contamination. The objective of the present study was to determine whether or not sufficient substrate would be present in feces and urine of pigs and dogs fed pyridine-N-oxides in rations to result in the production of detectable pyridine odors when the wastes were disposed of in soil. Analysis of lung gas was also done to determine whether or not either reduced or unreduced substrate was excreted via the breath. Gas chromatography was used for analysis of the substrate and the end-products in the excretory waste products and respiratory gases. Bacteriological analyses were conducted to determine whether or not consumption of the detector substrates caused changes in the intestinal flora of the experimental animals. The analytical data are discussed in terms of the study objectives and conclusions are made on the effects of the detector substrates in feces and urine.

Subject Categories:

  • Food, Food Service and Nutrition
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE