Thermoinactivation of Viruses. Communication IV: Factors Determining the Dynamics and Rate of Inactivation of Venezuelan Equine and Encephalomyelitis Virus (VEE)
FORT DETRICK FREDERICK MD
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The effect of a number of biological, chemical and physical factors on the course of the process of infectivity inactivation at different temperatures was studied on the model of VEE virus. Virus populations obtained in HeLa and MASHA cells were found to be less stable to heating at 50 degrees C than those obtained in cells of trypsinized embryos and RES cells. Ultracentrifugation resulted in reduced thermostability of VEE virus and increased its sensitivity of thermostabilizing effect of 12.5 magnesium sulphate. Decreasing of pH of the virus-containing suspension from 9.0 to 7.0 slightly slowed down thermoinactivation of VEE virus by the nucleic type more curde changes in pH markedly enhanced inactivation of the virus. In all cases, a change in the duration and dynamics of the process of themoinactivation of VEE virus occurred owing to a regular change of a limited number of mechanisms of the loss of infectious activity, the specific manifestations of which are discussed.