Thermoinactivation of Viruses: Report V. Thermoinactivation and Thermostabilizing Agents as a Factor Determining the Level of VEE Virus Production in Cell Cultures
FORT DETRICK FREDERICK MD
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The general productivity of cells in mediums of different compositions was studied on the model of Venezuelan equine encephalomyelitis virus VEE and trypsinized chick embryo cells. Medium 199 with 2 bovine serum provided long-term and active virus production, the total yield of virus particles per one cell exceeding 10,000 PFU. In minimal essential medium Earles solution with 0,22 sodium bicarbonate the total virus yield was more than 10 times lower despite the virus amplification to comparatively high titers. The most effective agents providing for adequate thermostabilization of VEE virus at 37 degrees C were found to be 2 bovine serum, 0,1 lactalbumin hydrolystate and 0,02 crystalline bovine albumin.