Chromatography of Cysteine and Glutathione Derivatives on a Dowex 1/Sephadex Mixed-Bed Column.
NATIONAL RESEARCH COUNCIL OF CANADA OTTAWA (ONTARIO) DIV OF BIOLOGY
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Dowex I X8 anion-exchange resin may be employed to separate many sulfinic and sulfonic acids which co-chromatograph on cation-exchange resins such as those normally employed in automatic amino acid analyzers. However, even on Dowex I X8, many important amino acids and peptides cochromatograph and we had difficulty while trying to measure sulfinic and sulfonic acid derivatives of cysteine and glutathione. Cysteic acid had the same elution time as glutathione disulfide which was always present in our samples. It was thought that addition of Sephadex G-10 to the resin bed would retard the smaller molecules enough to make a separation possible. Author