Drug Induced Ultrastructural Alterations of Plasmodium gallinaceum Oocysts in Aedes aegypti Mosquitoes.
Rept. no. 2 (Final),
LENOX HILL HOSPITAL NEW YORK DEPT OF PATHOLOGY
Pagination or Media Count:
Conventional techniques in electron were used microscopy to study drug changes in P. gallinaceum oocysts. Agent 49808 at the 0.1 level arrested oocyst development and produced damage to mitochondria including swelling and decreased matrix density. Pyrimethamine at the 0.001 level arrested oocyst development and prevented nuclear development and migration. Trimethoprim, 0.1, led to cytoplasmic vacuole formation and arrested oocyst development. Primaquine, 0.1, produces mitochondrial swelling and a decrease in matrix density in rare instances. Iso-pentaquine, 0.1, revealed a rare instance of dyssynchroney of oocyst differentiation in that a cytoplasmic structure usually seen at an advanced stage was noted in an otherwise undifferentiated oocyst. A drug withdrawal experiment utilizing 0.1 trimethoprim revealed that the drug arrests development at two to three days following the infective blood meal. Measurements of P. gallinaceum oocysts diameter treated with 0.001 pyrimethamine and 0.1 49808 when compared to a derived growth curve of untreated oocysts showed that these drugs also arrest P. gallinaceum oocysts at two to three days following the infective blood meal. These studies indicate that metabolically significant events occur at this time in the oocyst cycle. Virus-like particles are noted in P. gallinaceum oocysts treated with trimethoprim, pyrimethamine, primaquine and 49808. The possible significance of these particles is discussed. Author