Accession Number:

AD0730277

Title:

Lecithin Synthesis in Lungs.

Descriptive Note:

Final rept. 1 Apr 68-1 Apr 70,

Corporate Author:

IIT RESEARCH INST CHICAGO ILL

Report Date:

1971-07-01

Pagination or Media Count:

55.0

Abstract:

The objective was to study effects of oxygen exposure upon production and properties of lung surfactant. Compared with air-exposed controls, rabbits exposed to oxygen for 72 hr showed weight loss and an increase of approximately one pH unit in saline lung washings by in vitro lavage. Two phospholipid moieties were separated from the lavage by density-gradient centrifugation. Of these, the quantity of layer A top decreased to one-half, while layer B increased two- to four-fold upon oxygen exposure. Small differences were found in the distribution of specific fatty acids of layer A or layer B with oxygen exposure, resulting in a greater degree of unsaturation. Palmitate-1-14C specific activity appeared rapidly in lung lipids and more slowly in surfactant phospholipids both were decreased by oxygen exposure. Surfactant film-balance measurements showed non-surface-active contaminants in the lecithin isolated from layer B. Oxygen exposure resulted in marked changes in the surface properties of phospholipid fractions. Author

Subject Categories:

  • Biochemistry
  • Anatomy and Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE