Research in Human Blood Preservation by Freezing. Phase A. Studies on Reduction of Incidence of Post-Transfusion Hepatitis in Blood so Preserved. Phase B. The Development of Disposable Plastic Equipment to so Preserve Blood.
BLOOD RESEARCH INST INC BOSTON MASS CYTOLOGY LABS
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Incidence of posttransfusion hepatitis in previously frozen blood was significantly reduced by a transmembrane washing procedure. The Blood Research Institute method constitutes mechanical centrifugation, high glycerol concentration, slow freeze and thaw, and a 4-liter washing procedure for deglycerolization. Four cases of histologically proved hepatitis occurred in a control group of 104 recipients receiving 442 transfusions of cells resuspended in albumin p 0.029. The mechanism by which hepatitis virus is eluted or inactivated is not revealed by the study. A simplified method for automated red cell processing is described. The method uses sterile, disposable plastic centrifuge bowls with attached tubing and reagent solutions. It can be used for the separation of red cells and plasma and the glycerolization and deglycerolization of red cells for storage in the frozen state. Data is presented on operational performance of the equipment and on red cell yields and post-thaw transfusion survival. Author
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