The Effect of Laser Energy on Cells in Tissue Culture.
Annual summary rept. no. 3,
PASADENA FOUNDATION FOR MEDICAL RESEARCH CALIF DEPT OF LASER BIOLOGY
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The ultraviolet laser UVL produced different degrees of effects on cells of different types. Hamster DON-C cell populations were reduced in number following treatment, but recovered numerically, morphologically, and in rates of 3H-TdR uptake. Human malignant CMP cells did not show recovery. Human lung WI-38 cells showed loss of nucleolar and ribosomal components, while mitochondria were the most sensitive structure in CMP cells. Multiple exposures caused a reduction in DNA templace activity during RNA synthesis, and it inhibited rates of LDH catalysis. The UVL produced known types of chromosomal aberrations in DON-C cells, but human lymphocytes were primarily killed, but showed no aberrations in survivors. A concentration of 0.1 microgramml of quinacrine was not toxic, but could photosensitize nucleoli of CMP cells to the blue wavelengths from the argon laser. Mitochondria of myocardial cells contained natural chromophores, the cytochromes. Acridine orange-stained newt lung chromosomes showed both an 0.5 micron diameter paling and a loss of function following argon microbeam laser treatment. Recent studies showed a relationship between the nucleolar organizer and the mitotic process. Author