Accession Number:

AD0728029

Title:

Recovery of White Blood Cells After Freezing.

Descriptive Note:

Final rept.,

Corporate Author:

AMERICAN FOUNDATION FOR BIOLOGICAL RESEARCH MADISON WIS

Report Date:

1971-07-27

Pagination or Media Count:

20.0

Abstract:

Whereas the red cells recover from the actual congelation of all the water freezable at -3C, the neutrophils are already injured when only a fraction of the water freezable at -1.5C is congealed. Electron microscope studies of freeze-dried or freeze-subsituted suspensions of leucocytes indicated that freezing at rates varying from 30 to 2000 degrees C per second results always in the formation of intracellular and intranuclear ice in neutrophils. A study by concentrations of solutions of the cryoprotectants ethylene glycol, glycerol and polyvinyl pyrrolidone, undergo homogeneous nucleation throws some light on the conditions under which ice nuclei may originate in cryoprotected biological material. Author

Subject Categories:

  • Anatomy and Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE